Bayreuth 1998 – wissenschaftliches Programm
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CP: Chemische Physik
CP 18: Sondersymposium: Einzelmolekülspektroskopie
CP 18.2: Vortrag
Donnerstag, 12. März 1998, 11:50–12:10, H18
On the Track to Two Dimensional Single Molecule Detection of Biomolecules in Solution — •Harald Mathis, Geert Kalusche, and John McCaskill — Institut f"ur Molekulare Biotechnologie e.V., Beutenbergstra"se 11, D-07745 Jena
Spatial cross-correlation of photons is better suited to the
characterization of the trajectories of biomolecules than
single channel fluorescence correlation spectroscopy.
The limiting case of full spatial imaging with the necessary
temporal resolution to allow single photon cross-correlation
would be the method of choice. Developments in x-y-strip
detectors from particle physics and MHz gatable
multi-channel-plate image intensifiers when combined with parallel
configurable hardware offer the possibility to extend single or few
channel single molecule detection to two dimensions, even for
molecules in free solution.
In this contribution first results with the new detector
will be presented and the principal benefits and limitation
of this approach compared with APD arrays discussed. Potential
applications include molecular sorting size characterization
(in free solution and in gel) and observation of stochastic
chemical kinetics. The results of the zero-dimensional single
molecule detection with an open confocal setup will be presented.
We will show, that DNA and RNA oligonucleotides < 100 bp are
detectable in microstructures when labelled merely by
reversible binding intercalators such as Thiazol Orange and
its derivatives.