Münster 1999 – scientific programme

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CP: Chemische Physik

CP 32: Poster: Einzelteilchenspektroskopie

CP 32.1: Poster

Monday, March 22, 1999, 18:00–20:00, R52/R72

Single Molecule Imaging by 2-Photon Excitation — •M. Sonnleitner, G.S. Harms, G.J. Schütz, and Th. Schmidt — Institute of Biophysics, University of Linz, 4040 Linz, Austria

Wide-field two-photon excitation was exploited to study the diffusion of individual fluorescence-labeled lipids in a supported phospholipid membrane on a millisecond time scale. A CCD-camera was used for direct imaging of individual molecules.

The two-photon approach allowed for low background parallel observation up to a field-of-view of 5µ m size. The signal-to-noise ratios are compared to conventional one-photon measurements in terms of excitation wavelength, laser intensity, and illumination time.

Supported by the Austrian Research Fonds, Project P12803-MED