Berlin 2001 – wissenschaftliches Programm

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CPP: Chemische Physik und Polymerphysik

CPP 1: Special Symposium: Molecular Dynamics in Confined Geometries

CPP 1.4: Fachvortrag

Montag, 2. April 2001, 12:30–13:00, 3010

Stimulating, Manipulating, and Probing the Mechanical Behaviour of single GFP-Actin Fibroblast Cells on Nanostructured Surfaces — •Joachim Spatz¹, Alexandre Micoulet¹, Marcell Ott¹, Albrecht Ott², and Martin Möller³ — ¹Universität Heidelberg, Biophysikalische Chemie, Institut für Physikalische Chemie, INF 253, 69120 Heidelberg, E-Mail: Joachim.Spatz@urz.uni-heidelberg.de — ²Institut Curie, Paris, France — ³Universität Ulm, Organische Chemie III / Makromolekularechemie

The chemical-physical properties of synthetic macromolecules and peptides have been used to modify nanostructured surfaces on the molecular level. These surfaces were used to manipulate the adhesion and motility properties of GFP-actin fibroblast cells based on single receptors allowing the cells to be probed with a pattern made of individual receptors. The interaction of cells with such surfaces were studied by applying a uniaxial force to an individual cell. Conditions of constant force or constant deformation allowed the determination of the dynamic reaction of a cell to an external, mechanical stimulation. By using GFP-actin fibroblast cells, the dynamic development of actin filaments were monitored in-situ during defined external stimulation.