Bereiche | Tage | Auswahl | Suche | Downloads | Hilfe

SYBP: Biophotonik

SYBP 1: Einzelmolekülmethoden I

SYBP 1.2: Hauptvortrag

Donnerstag, 7. März 2002, 11:30–12:00, HS 11/215

Ultrasensitive Microscopy to Image Molecular Processes in Living Cells — •Gerhard Schütz — Inst.f. Biophysics, Johannes-Kepler-University Linz, Austria

A detailed understanding of molecular processes is the basic requirement for the description of cellular function. These processes typically involve the interplay of different proteins, but also of proteins and the lipids in the cell membrane. While large scale rearrangements of the protein distribution proceed on seconds up to minutes time scales, local changes are much faster. New ultra-sensitive methodologies for imaging single molecules in living cells allow the direct observation of molecular rearrangements on millisecond time scales. Since molecular movements in general proceed in three dimensions, an apparatus for rapid recording of the three dimensional position of single biomolecules is often required. In this presentation, studies of the mobility and interaction kinetics of individual molecules in living cells will be described. First examples of 3D imaging of single molecules demonstrate the scope of this approach.