Regensburg 2002 – scientific programme
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SYBM: Physik biologischer Materie
SYBM 202: Spectroscopy of single biomolecules
SYBM 202.1: Talk
Tuesday, March 12, 2002, 15:00–15:15, H37
Single Virus Tracing: Infectious Entry Pathway of a Single Dye-Labeled Virus into a Living Cell — •Thomas Endreß1, Stefan Grimm1, Georg Seisenberger1, Hildegard Büning2, Michael Hallek2, and Christoph Bräuchle1 — 1Department Chemie, Lehrstuhl Physikalische Chemie I and Center for Nanoscience, Ludwig-Maximilians-Universität München, Butenandtstr. 11, 81377 München, Germany — 2Genzentrum of the Universität München, Feodor-Lynen-Str. 25, 81377 München, Germany
Single molecule detection is a novel technique for the investigation of biological processes on the molecular level. Here we show for the first time that this method can be used for the visualisation of the infectious entry pathway into a living cell. Only one fluorescent dye molecule (Cy5 or Cy3 respectively) was attached to a virus in order not to influence its physiological behavior. The fluorescent dye-label allows one to observe the movement of a virus.
A detailed picture of the processes involved in the uptake of a virus in a living cell could be obtained. This may be essential for the development of antiviral drugs and gene therapy vectors.