Regensburg 2002 – scientific programme
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SYBM: Physik biologischer Materie
SYBM 401: Physics of biological matter: Poster
SYBM 401.12: Poster
Tuesday, March 12, 2002, 18:00–19:30, H37
Mechanics, Stress Fiber Formation and Force Generation of a single GFP-actin Fibroblast — •Alexandre Micoulet1, Joachim Spatz1, and Albrecht Ott2 — 1University of Heidelberg, Institute of Physical Chemistry, Biophysical chemistry, Im Neuenheimer Feld 253, D-69120 Heidelberg — 2University of Bayreuth, Chair of Experimental Physics I, D-95440 Bayreuth
Differentiation and genetic expression of cells as well as development of first embryonic states are constantly regulated processes where biochemical and mechanical interactions between a cell and its environment play a major role. We want to stimulate a cell by defined biochemical receptors and external mechanical stress. The chemical-physical properties of synthetic macromolecules and peptides have been used to modify surfaces on the molecular level. These surfaces were used to manipulate the adhesion and motility properties of GFP-actin fibroblast cells. The interaction of cells with such surfaces were studied by applying a uniaxial force to an individual cell. Conditions of constant stress or constant strain allowed the determination of the dynamic reaction of a cell to an external, mechanical stimulation. By using GFP-actin fibroblast cells, the dynamic development of actin filaments were monitored in-situ during defined external stimulation.