Regensburg 2002 – scientific programme

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SYBM: Physik biologischer Materie

SYBM 401: Physics of biological matter: Poster

SYBM 401.31: Poster

Tuesday, March 12, 2002, 18:00–19:30, H37

Kinetic and Thermodynamic Analysis of ssDNA-RPA Interactions with SPR and FCS — •F. Schubert¹, H. Zettl¹, G. Krauss², and G. Krausch¹ — ¹Physikalische Chemie II, Universität Bayreuth, 95447 Bayreuth — ²Laboratorium für Biochemie, Universität Bayreuth, 95447 Bayreuth

The interactions of immobilized single-stranded ARS121-1 DNA and replication protein A (RPA) were observed by surface plasmon resonance (SPR) using a BIACORE X. The analysis included the determination of the association rate constant k_a, the dissociation rate constant k_d and the equilibrium constant K_D. From this the Gibbs free energy Δ G and the transition state free energy Δ G^‡ were calculated. By measuring the reaction at different temperatures, it was possible to extract thermodynamic information like Δ H and Δ S about the system using van’t Hoff analysis.

The kinetic and thermodynamic information gained by SPR were compared with data observed by fluorescence correlation spectroscopy (FCS).