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SYBM: Physik biologischer Materie

SYBM I: HV I

SYBM I.1: Hauptvortrag

Dienstag, 12. März 2002, 09:30–10:15, H37

Single Molecule Study of a Helicase — •David Bensimon — Laboratoire de Physique Statistique, ENS, Universites Paris VI and VII, Paris

The activity of a single helicase opening a double strand DNA can be monitored in real time using a magnetic trap technique to pull on a single DNA molecule and observe its increase in extension as it is unwound into two ssDNAs. This observation allows one to measure the rate of unwinding, the enzyme’s processivity, on-time and step size as a function of force and ATP concentration. These single molecule measurements differ markedly from bulk measurements of the activity of the helicase we study. We shall explain why.