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CPP: Chemische Physik und Polymerphysik
CPP 10: Experimente mit Einzelmolekülen
CPP 10.6: Vortrag
Donnerstag, 27. März 2003, 11:15–11:30, ZEU/160
Single molecule level detection of hydrophobicity change during GroEL activity by Atomic Force Microscopy — •Giovanni Dietler1, Francesco Valle1, James A. DeRose1, Gerit Kulik1, Martin Kawe2, and Andreas Plückthun2 — 1Inst. of Condensed Matter Physics, University of Lausanne, CH-1015 Lausanne, Switzerland — 2Biochemisches Institut der Universität Zürich, CH-8057 Zürich, Switzerland
GroEL is a protein complex with a toroidal shape and a central cavity. GroEL, a chaperonin molecule, folds misfolded or newly synthetized proteins. The activity cycle of GroEL implies a change of the central cavity from hydrophobic to hydrophilic character. By means of the Atomic Force Microscopy it was possible to show at the single molecule level that the central cavity changes from hydrophobic to hydrophilic during the ATP cycle. This measurement was carried out by exploiting the attractive or repulsive interaction between the GroEL cavity and the AFM tip.