Dresden 2003 – wissenschaftliches Programm

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CPP: Chemische Physik und Polymerphysik

CPP 22: POSTER C

CPP 22.24: Poster

Donnerstag, 27. März 2003, 12:00–14:00, ZEU/250

Implementation of a Cryogenic Infrared Single-Molecule Microscope based on Total Internal Reflection. — •Jürgen Baier, Martin Richter, Clemens Hofmann, and Jürgen Köhler — Experimental Physics IV, University of Bayreuth

Visualization of individual molecules requires an efficient suppression of elastically scattered photons. Currently widefield- and/or confocal microscopy are the predominantly used techniques to achieve this goal. In widefield-imaging many molecules are imaged onto a detector and can be observed at the same time. However, the signal-to-noise ratio is not optimum due to the large background arising from the excitation light. In contrast, confocal microscopy offers a high background reduction at the cost of the sequential character of the method. Total internal reflection (TIR) microscopy combines the advantages of both methods. It is a widefield technique that allows parallel recording of single molecules with superior background supression. Here, we report the implementation of a infrared microscope based on TIR operating at cryogenic temperatures.