München 2004 – scientific programme

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Q: Quantenoptik und Photonik

Q 43: Biophotonik und Laser in der Medizin

Q 43.3: Talk

Thursday, March 25, 2004, 17:00–17:15, HS 224

Two Photon Fluorescence Spectroscopy in Physiological Tissue — •Christian Spitz and Ralf Menzel — Universität Potsdam, Institut für Physik, Am Neuen Palais 10, 14469 Potsdam

The fluorescence of endogeneous fluorophores as NADH, FAD, and Protoporphyrin IX is a useful tool in research and clinical diagnostics as these molecules act as markers for metabolism activity. In physiological samples unfortunately the strong autofluorescence from connective tissue, namely Collagen and Elastin, becomes a problem under UV illumination.

We can show how this problem can be overcome by two photon excitation with fs-laserpulses in the near infrared. Under this excitation conditions the fluorescence behaviour of Collagen and Elastin drastically changes while the aimed Fluorophores act as under single photon excitation.