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AKB: Biologische Physik

AKB 50: Poster Session "Biological Physics"

AKB 50.117: Poster

Freitag, 12. März 2004, 10:30–13:00, B

Cell Adhesion onto Highly Curved Particle Surfaces - One-Step Immobilization of Cell Membranes — •Motomu Tanaka und Stefan Kaufmann — Biophysics Lab, Tech. Univ. Munich

We report single-step, orientation selective immobilization of human erythrocyte membranes on bare silica beads with different topographies: 1) solid (nonporous) silica beads with a diameter of 3 microns and 2) porous silica beads with a diameter of 5 microns. Erythrocyte membranes were immobilized onto beads simply by incubation, without sonication or osmotic lysis. Membrane orientation before and after the immobilization was identified with a first monoclonal antibody and a second fluorescent polyclonal antibody. Adherent erythrocytes on the beads all ruptured, inverted the asymmetric orientation of the membrane, and selectively exposed their cytoplasmic domain. The surface topography did not influence orientation or the amount of immobilized membranes. On the other hand, the fact that no adsorption or rupture of erythrocytes could be observed on planar quartz substrates suggests a significant influence of contact curvatures on the adhesion free energy.