Regensburg 2004 – wissenschaftliches Programm
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AKB: Biologische Physik
AKB 50: Poster Session "Biological Physics"
AKB 50.120: Poster
Freitag, 12. März 2004, 10:30–13:00, B
Functional Micro-Domains of Glycolipids with Partially Fluorinated Membrane Anchors - Impact on Cell Adhesion — •Motomu Tanaka1, Matthias Schneider1, Laurent Limozin1, Doris Heinrich1, Gabriele Schumacher2, Gerd Bendas2, Ulrich Rothe3, Christian Gege4, and Richard Schmidt4 — 1Dept. Physics E22, Tech. Univ. Munich — 2Dept. Pharmacy, Univ. Bonn — 3Dept. Physiol. Chem., Univ. Halle — 4Dept. Chem., Univ. Konstanz
Functional micro-domains of glycolipids were designed by mixing neoglycolipids with partially fluorinated (F-alkyl) chains and matrix lipids with alkyl chains. Fluorescence images of the mixed lipid monolayers at air/water interface demonstrated that it is possible to control both size and distribution of the micro-domains according to the strong de-mixing of alkyl and F-alkyl membrane anchors, while the correlation between carbohydrate head groups seemed to play rather minor roles. These micro-domains in monolayers could be transferred onto hydrophobized substrates, and subjected to dynamic flow chamber experiments. The results obtained here clearly indicated that the dynamic adhesion of Chinese hamster ovarial cells expressing E-selectin (CHO-E cells) onto the lipid monolayer containing micro-domains of sialyl LewisX (sLeX) can be both enhanced and reduced by controlled de-mixing of ligands and matrices. Moreover, the same clusters of sLeX could also be formed in giant lipid vesicles, which can be used as a model cell that locally expresses bio-specific functions.