Regensburg 2004 – scientific programme
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AKB: Biologische Physik
AKB 50: Poster Session "Biological Physics"
AKB 50.129: Poster
Friday, March 12, 2004, 10:30–13:00, B
Functional Incorporation of Cell Receptors into Polymer Tethered Membranes - Influence of Spacer Length and Density — •Oliver Purrucker1, Stephanie Gönnenwein1, Monika Rusp1, Anton Förtig2, Rainer Jordan2, and Motomu Tanaka1 — 1Technische Universität München, Lehrstuhl für Biophysik E22, James-Franck-Str., D-85748 Garching — 2Technische Universität München, Lehrstuhl für Makromolekulare Stoffe, Lichtenbergstr. 4, D-85748 Garching
To study physical principles of cell-cell interactions, supported lipid membranes with cell surface receptors have been intensively and widely investigated as a general model of plasma membranes. The close proximity of the artificial membrane to the solid support (0.5 - 2 nm) does not provide a sufficient water reservoir that even causes nonspecific adsorption and denaturing of proteins. This problem can be overcome by introduction of hydrophilic polymer spacers, which can provide more No-dqfluidNo-dq environments for proteins. In our recent study, we designed a new type of polymer-tethered membrane with defined poly(2-methyl-2-oxazoline) lipopolymer spacers and could demonstrate the incorporation of transmembrane cell receptors1.
Measurements of adhesion free energy of a ligand-doped lipid vesicle on the supported membrane with integrin αIIbβ3 receptors, we found that the functionality of incorporated integrin receptors systematically depends on the density and length of the lipopolymer tethers.
[1] O. Purrucker, A. Förtig, R. Jordan, M. Tanaka, ChemPhysChem in print