Regensburg 2004 – wissenschaftliches Programm
Bereiche | Tage | Auswahl | Suche | Downloads | Hilfe
AKB: Biologische Physik
AKB 50: Poster Session "Biological Physics"
AKB 50.13: Poster
Freitag, 12. März 2004, 10:30–13:00, B
Artificial actin cortices on microfabricated pillar arrays — •Wouter Roos1, Alexander Roth2, Roman Glass1, Erich Sackmann2, and Joachim P. Spatz1 — 1Universitaet Heidelberg, Institut fuer physikalische Chemie, Biophysikalische Chemie, 69120 Heidelberg — 2Technische Universitaet Muenchen, Physik-Department E22, 85747 Garching
Arrays of microfabricated pillars are constructed to serve as a template for mimicking the actin cortex. Different methods to fabricate pillar arrays will be discussed, these involve top-down and bottom-up approaches using photolithographic techniques, plasma etching processes and epitaxial ZnO growth. A two-dimensional network of actin filaments, that is pending from the pillar tops, is fabricated. Due to the 3-dimensional template surface interaction of the filaments hanging in between the pillars with substrate surfaces is prevented. This opens new possibilities to study the mechanics of 2-dimensional actin networks as a function of actin-crosslinkers, and the active diffusion of molecular motors operating on pending networks. The behaviour of this artificial actin cortex will be compared to models of networks and to cortices in living cells.