Regensburg 2004 – wissenschaftliches Programm

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AKB: Biologische Physik

AKB 50: Poster Session "Biological Physics"

AKB 50.33: Poster

Freitag, 12. März 2004, 10:30–13:00, B

Application of micron and nano scale metal island patterns as coordinative system for quantifying intracellular diffusion — •Tamas Haraszti and Joachim P. Spatz — University of Heidelberg, INF 253, 69120 Heidelberg

Quantitative determination of intracellular diffusion in adhering cells allows access to evaluate dynamics important in cell functions. On very small length scales we face the problem that the absolute localisation of a diffusive probe such as fluorescent molecules or quantum dots in cells relative to the adhering substrate might fail. In our studies we correlate cellular diffusion to an absolute coordinative system made of micro and nano scale metal islands. Either e-beam or micellar diblock copolymer lithography applied to form the desired patterns. The patterns are identified by statistically distributed or alternating gold and silver islands. A microscope with ATR capability allows to evaluated both, the coordinate system and the diffusion of molecular probes in cells.