Regensburg 2004 – scientific programme

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AKB: Biologische Physik

AKB 50: Poster Session "Biological Physics"

AKB 50.36: Poster

Friday, March 12, 2004, 10:30–13:00, B

Functionalization and Passivation of a Silicon-on-Insulator based Sensor Device — •Petra A. Neff, Michael G. Nikolaides, Stefan Rauschenbach, Simon Q. Lud, and Andreas R. Bausch — Lehrstuhl für Biophysik - E22, Technische Universität München, 85747 Garching

The sensitive and specific detection of biomolecular interactions relies on the functionalization and passivation of the detecting surfaces. Recently, a new Silicon-on-Insulator (SOI) based thin film resistor device for chemical and biological sensor applications was introduced. It has been shown that this sensor is highly sensitive to variations of the surface potential evoked by the adsorption of small amounts of charged molecules. Whereas most of the conventional detection techniques require the labeling of molecules, the SOI based sensor allows a label-free detection of interactions.

Here we discuss the passivation and functionalization of the bare silicon oxide surface by physical adsorption of polyelectrolytes and the covalent binding of silanes. Results of the adsorption of PAH/PSS multilayers are presented and their interpretation will be discussed. First results of the application of the sensor device towards the label-free detection of DNA hybridization will be presented.