Regensburg 2004 – wissenschaftliches Programm
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AKB: Biologische Physik
AKB 50: Poster Session "Biological Physics"
AKB 50.37: Poster
Freitag, 12. März 2004, 10:30–13:00, B
Activation of Integrin Function by Nanopatterned Adhesive Interfaces — •Marco Arnold1, Elisabetta Ada Cavalcanti 1, Roman Glass1, Jacques Blümmel1, Wolfgang Eck2, Martin Kantlehner3, Horst Kessler3, and Joachim P. Spatz1 — 1University of Heidelberg, Institute for Physical Chemistry, Biophysical Chemistry, INF 253, D-69120 Heidelberg — 2University of Heidelberg, Institute for Physical Chemistry, Applied Physical Chemistry, INF 253, D-69120 Heidelberg — 3Technical University of Munich, Institute for Organic Chemistry and Biochemistry, Lichtenbergstrasse 4, D-85747 Garching
To study the function behind molecular arrangement of single integrins in cell adhesion, we designed a hexagonally closepacked rigid template of cell adhesive gold nanodots by lithographic means of diblock copolymer selfassembly. The diameter of the adhesive dots are smaller than 8nm, which allows the binding of one integrin per dot. These dots are positioned with high precision at 28, 58, 73 and 85nm spacing at interfaces. A separation of more than 73nm between the dots results in limited cell attachment and spreading and reduces the formation of focal adhesion and actin stress fibers. We attribute these cellular responses to restricted integrin clustering rather than insufficient number of ligand molecules in cellmatrix interface since micronanopatterned substrates consisting of alternating fields with dense and no nanodots support cell adhesion. We propose that the range between 58 and 73nm is a universal length scale for integrin clustering and activation.