Regensburg 2004 – wissenschaftliches Programm
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SYLS: Life Sciences on the Nanometer Scale - Physics Meets Biology
SYLS 3: Symposium "Life Sciences on the Nanometer Scale - Physics Meets Biology"
SYLS 3.14: Poster
Mittwoch, 10. März 2004, 16:00–18:30, B
Optimizing Water-Soluble Quantum Dots for Biological Application — •Vladimir V. Breus1, Colin D. Heyes1, Andrei Yu. Kobitski1, Kirill V. Anikin 1, and G. Ulrich Nienhaus 1,2 — 1Department of Biophysics, University of Ulm, D - 89069 Ulm, Germany — 2Department of Physics, University of Illinois Urbana-Champaign, Urbana, IL 61801, USA
The use of quantum dots has several advantages over fluorescent dyes for biological application. They are bright, easy tunable in color, and extremely stable against photo bleaching (>108 emitted photons). Various bifunctional ligands were used to obtain water-soluble, biocompatible ZnS coated CdSe quantum dots. The chemical stability, photoluminescence efficiency and fluorescent blinking of the different samples were compared. The optimized water-soluble quantum dots were used in long-timescale single-molecule imaging of lipid diffusion in membranes.