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SYLS: Life Sciences on the Nanometer Scale - Physics Meets Biology

SYLS 3: Symposium "Life Sciences on the Nanometer Scale - Physics Meets Biology"

SYLS 3.27: Poster

Mittwoch, 10. März 2004, 16:00–18:30, B

Controlled proliferation of living cells on UV-light modified polymers — •Thomas Gumpenberger¹, Johannes Heitz¹, Dieter Baeuerle¹ und Christoph Romanin² — ¹Angewandte Physik, Universitaet Linz, Austria — ²Biophysik, Universitaet Linz, Austria

We demonstrated the controlled proliferation of human umbilical endothelial cells (HUVEC) on UV-light modified polymer samples. The polymers under investigation were either polytetrafluoroethylene (PTFE) or polyethyleneterephtalate (PET), which are among the most frequently employed biomaterials in reconstructive medicine. The PTFE surfaces were modified by exposure to the ultraviolet (UV) light of a Xe2*-excimer lamp at a wavelength of 172 nm in an ammonia atmosphere. The irradiation led to an efficient exchange of the F-atoms in the surface by other chemical moieties. In-vitro, this resulted in a significant increase in the number of adhering cells 1 day after seeding and in the formation of a confluent cell layer after 3 to 8 days. The results were comparable or even better than those obtained on standard polystyrene petri-dishes used in cell cultivation. Similar studies were performed on PET.