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SYLS: Life Sciences on the Nanometer Scale - Physics Meets Biology

SYLS 3: Symposium "Life Sciences on the Nanometer Scale - Physics Meets Biology"

SYLS 3.40: Poster

Mittwoch, 10. März 2004, 16:00–18:30, B

Single cell manipulation in microfluidic networks by optical tweezers — •Kai Leffhalm¹, Andy Sischka¹, Wibke Hellmich¹, Thanh Tu Duong¹, Annika Grabbe², Jürgen Wienands², Katja Tönsing¹, Robert Ros¹, Alexandra Ros¹, and Dario Anselmetti¹ — ¹Experimental Biophysics, Physics Department, Bielefeld University, Germany — ²Biochemistry, Department of Chemistry, Bielefeld University, Germany

Experiments with single cells gain importance as a tool to better understand the behaviour of living cells in vivo. Microfluidic networks provide dimensions small enough to navigate a single cell with optical tweezers to different areas of the artificial network where the flow properties can be controlled by electrophoresis and electroosmosis.

The expression of special proteins in a cell can be stimulated or suppressed by changing the velocity of the flow or the concentration of substances, e.g. cytokines or (cytostatic) drugs, in the culture medium.

We will present our experimental setup and our first test experiments for future applications in monitoring the expression level of individuell cells and microproteomics.