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SYLS: Life Sciences on the Nanometer Scale - Physics Meets Biology

SYLS 3: Symposium "Life Sciences on the Nanometer Scale - Physics Meets Biology"

SYLS 3.50: Poster

Mittwoch, 10. März 2004, 16:00–18:30, B

Composition and Decomposition of DNA-Containing Polyelectrolyte Multilayers — •Rolf Dootz¹, Jingjing Nie¹, Binyang Du¹, Alexander Otten¹, Wolfgang Schnitzler¹, Stephan Herminghaus^1,2 und Thomas Pfohl¹ — ¹Angewandte Physik, Universität Ulm, Albert-Einstein-Allee 11, 89081 Ulm — ²Max-Planck-Institut für Strömungsforschung, Bunsenstrasse 10, 37073 Göttingen

The layer-by-layer technique provides a convenient and controllable way of forming functional and organised polyelectrolyte multilayer films. In this study multilayers consisting of DNA and polypropyleneimine dotriacontaamine dendrimer, generation 4.0 (G4), which are discussed for nonviral gene delivery systems, have been investigated. A nonlinear growth of film thickness with increasing layer number has been observed. A growth mechanism of polyelectrolyte multilayer based on charge overcompensation during the buildup process is proposed, which is able to explain the nonlinear growth. Furthermore, combined addition of mono- and multivalent ions leads to a controlled decomposition of the layers. The release of embedded DNA-molecules may be tuned by varying the concentration of the salts.