Berlin 2005 – wissenschaftliches Programm

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AKB: Biologische Physik

AKB 10: Biopolymers

AKB 10.1: Hauptvortrag

Freitag, 4. März 2005, 09:45–10:15, TU H2013

Interfacial Forces move DNA in Thermal Gradients — •Dieter Braun — Noether Group on Dissipative Biosystems, Applied Physics, LMU München, Amalienstr. 54, D-80799 München

Temperature gradients can induce large effects at microscopic dimensions. We discuss the first measurement of thermophoresis of DNA [1] and show how this effect can be used to accumulate and separate Microparticles or DNA [1-3]. Within microfluidic environments, the field gradients can be applied freely by IR optics. Finite element methods allow the in-depth simulation of thermophoresis, thermal conduction and fluid flow to compare with experiments even in complicated settings. We discuss measurement approaches and applications of thermophoresis, show how thermophoresis can complement our new approach of convective PCR [4] and speculate whether the effect might have played a role in molecular evolution [5].

[1] Trapping of DNA by Thermophoretic Depletion and Convection, Physical Review Letters 89: 188103 (2002)

[2] Thermophoresis of DNA determined by Microfluidic Fluorescence, European Physical Journal E, in press

[3] 2D Colloidal Crystals formed by Thermophoresis and Convection, in preparation

[4] Exponential DNA Replication by Laminar Convection, Physical Review Letters 91: 158103 (2003)

[5] Thermal force approach to molecular evolution, Physical Biology 1: P1-P8 (2004)