Berlin 2005 – scientific programme
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AKB: Biologische Physik
AKB 100: Poster Session I
AKB 100.27: Poster
Saturday, March 5, 2005, 16:45–18:45, Poster TU D
Proteins under shear stress on planar surfaces — •Stefan Nuschele1, Stefan W. Schneider2, Matthias F. Schneider1, and Achim Wixforth1 — 1Universität Augsburg, Experimentalphysik1, Biophysik, Universitätsstr. 1, 86135 Augsburg — 2Universität Münster, Abteilung Dermatologie, Von-Esmarch-Str. 56, 48149 Münster
Von-Willebrand-Factor (vWF) forms large fibers and mediates binding of blood platelets to the vascular endothelium at wounded and inflamed tissue. The released and activated protein undergoes a coil to fiber transition. In vivo among other things this procedure presumably is induced by blood shear flow. A dysfunction causes blood clotting diseases. By means of surface acoustic waves (SAW) we mimic the blood flow using a novel designed bio-chip. The blood vessels are imitated in two dimensions by hydrophilic channels in hydrophobic surface surrounding. The SAWs, adjustable in power, cause laminar flow. Using fluorescence microscopy we could proof the proposed model of activating the vWF. The combination of surface acoustic waves with flat fluidics and adapted surface structures enables new approaches to hemodynamic phenomena in vitro with reduction in sample volume to only a few µl.
Schneider at al. (2004) submitted APL