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Berlin 2005 – scientific programme

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AKB: Biologische Physik

AKB 100: Poster Session I

AKB 100.31: Poster

Saturday, March 5, 2005, 16:45–18:45, Poster TU D

Dynamics of individual actin filaments in extensional flow — •Dagmar Steinhauser1, Sarah Köster1, Wolfgang Schnitzler2, and Thomas Pfohl1,21Max-Planck-Institut für Strömungsforschung, Bunsenstraße 10, 37073 Göttingen — 2Angewandte Physik, Universität Ulm, Albert-Einstein-Allee 11, 89081 Ulm

We are interested in the dynamics of biopolymers under extensional flow and the formation of condensed structures, bundles and networks induced by inter-chain and intra-chain linker molecules. Therefore, individual F-actin filaments were investigated in hydrodynamic focusing devices by means of fluorescence microscopy. The microfluidic devices were fabricated by using soft lithography and a stroboscopic laser light illumination was applied for imaging the biopolymers in a continuous flow. Adding actin binding proteins such as α-actinin or filamin, the dynamic of the association, bundling, and network formation of a few individual molecules can be observed.

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