Berlin 2005 – wissenschaftliches Programm
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AKB: Biologische Physik
AKB 100: Poster Session I
AKB 100.39: Poster
Samstag, 5. März 2005, 16:45–18:45, Poster TU D
Biomimetic adhesion studies on chemically biofunctionalized nanostructures — •Christine Selhuber1, Irina Slizskaja1, Nadine Walter1, Fabian Czerwinski1, Jaques Blümmel1, and Joachim Spatz1,2 — 1Universität Heidelberg, Biophysikalische Chemie, INF 253, 69120 Heidelberg — 2Max-Planck-Institut für Metallforschung, Stuttgart
Exploring the physics of cell adhesion is essential for a detailed understanding of highly complex biological processes involved in cell-cell or cell-tissue interactions.
We focus on experimental studies of adhesion as a function of ligand density and pattern architecture. Functionalized nanostructures from self-assembled diblock copolymers represent an ideal platform to vary these parameters. The nanostructures are described by hexagonal patterns of nanometer sized gold dots where location and separation of single dots can be precisely controlled over a wide length scale. Functionalization of gold nanopatterns with streptavidin provides an adhesive model interface for biotinylated probes.
To extract the different physical contributions to adhesion on nanostructures we make use of two biomimetic model systems: On the one hand we are using biotin-covered elastic beads for measuring their adhesion induced deformation. This deformation can be related to surface energy and is a first parameter of adhesive strength. On the other hand, biotin-containing vesicles are a well-established tool to investigate adhesion kinetics. This is especially the case since it enables to study adhesion cluster stability by application of external forces.