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AKB: Biologische Physik

AKB 100: Poster Session I

AKB 100.69: Poster

Samstag, 5. März 2005, 16:45–18:45, Poster TU D

Merging Microfluidics and Optical Tweezers: a versatile Microtechnology Platform — •Christian Schmitz^1,2, Kai Uhrig^1,2, Jennifer Curtis^1,2, and Joachim Spatz^1,2 — ¹Max-Planck-Institut für Metallforschung, Stuttgart — ²Universität Heidelberg, Biophysikali-sche Chemie, Heidelberg

Microfluidic devices offer unique advantages in handling of small sample volumes. This is particularly important in bioanalysis and biotechnology. Furthermore, the miniaturization of flow systems leads to remarkably improved performance in separation and detection of reagents. As a step towards a more flexible and versatile system design we created a new technology platform by merging the techniques of holographic optical tweezers (HOTs) and microfluidics. HOTs can produce and independently steer one to hundreds of optical traps. It provides the possibility to non-invasive assembly and manipulation of micrometer size objects as well as to measure forces. The scale at which the microtechnology platform operates allows complete spatial and chemical control of the microenvironment und thus for the study of biological and biomimetic systems at a cellular level. We apply the system for probing complex biopolymer networks: F-Actin networks are engineered and their physical properties are studied as actin cortex model systems.