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AKB: Biologische Physik

AKB 200: Poster Session II

AKB 200.13: Poster

Dienstag, 8. März 2005, 17:00–19:00, Poster TU C

NAD(P)H autofluorescence - an approach to cellular metabolism — •Bülent Peker, Raluca Niesner, and Karl-Heinz Gericke — IPC @ TU-Braunschweig, Hans-Sommer-Str. 10, D-38106 Braunschweig

The 2-Photon-excitation based Fluorescence Lifetime Imaging (FLIM) proved to be an excellent method for subcellular research in biological samples in the last few years. It is now a seminal method for "ex vivo" and "in vivo" non invasive visualisation research in extensive united cell structure and tissue with high resolution. The options given by these method like monitoring a reply to a stimulus were unfortunately deadlocked by too long data interpretation. Our technique of FLIM-Analysis allows an on-line monitoring by implementing a non-iterativ method. In addition to multiexponential NAD(P)H- Analysis successful applications in pH-, η- and τ-imaging in artificial skin constructs show the capability of our method.