Berlin 2005 – scientific programme

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AKB: Biologische Physik

AKB 200: Poster Session II

AKB 200.14: Poster

Tuesday, March 8, 2005, 17:00–19:00, Poster TU C

Subcellular parameter probing using TPM based FLIM — •Stefan Quentmeier, Raluca Niesner, Bülent Peker, and Karl-Heinz Gericke — IPC @ TU-Braunschweig, Hans-Sommer-Str. 10, 38106 Braunschweig

Two-photon scanning microscopy (TPM) combined with fluorescence lifetime imaging (FLIM) provides an excellent method for probing cellular parameters on subcellular level. Depending on the dye used different parameters like pH, ionic strength, CO₂ and O₂ concentration and viscosity can be monitored in high resolution. FLIM gives us a non-invasive technique at hand possessing high intrinsic 3D resolution, large penetration depth, low photodamage and simple experimental setup as sample preparation is limited to simple staining. As fluorescence lifetime is not affected by experimental parameters the instrumental stability of standard intensity based TPM experiments is easily outperformed by FLIM. We performed FLIM for pH, n and viscosity mapping in artificial skin constructions (ASC) and genuine human skin.