Berlin 2005 – wissenschaftliches Programm

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AKB: Biologische Physik

AKB 200: Poster Session II

AKB 200.23: Poster

Dienstag, 8. März 2005, 17:00–19:00, Poster TU C

Use of carboxylic acids for the monitoring of anaerobic fermentation processes — •Dieter F. Ihrig¹, H. Michael Heise², Ulrich Brunert^1,2, Alexander Moor², Ruediger Kuckuk², and Martin Poschmann¹ — ¹University of Applied Sciences Suedwestfalen, Iserlohn, Germany — ²ISAS - Institute for Analytical Sciences, Dortmund, Germany

We are studying anaerobic fermentation processes that involve a thermophilic first bioreactor stage and a mesophilic second stage. The developed anaerobic process is very efficient, but also rather unstable. For achieving a better process management, it is necessary to understand the interdependencies between process engineering parameters (for example, the hydraulic turn-over time or the organic biomass burden as characterized by the Chemical Oxygen Demand (COD) parameter) and biochemical variables such as the concentration of carboxylic acids. Results from the determination of carboxylic acids using steam extraction and Reversed-Phase-HPLC are discussed. Furthermore, activities were started using infrared spectroscopy for quasi-continuous process monitoring. Goal of the project is the development of an on-line sensor system based on infrared attenuated total reflection (ATR) measurements. For gathering practical experience, we constructed a micro-flow system for on-site spectroscopic measurements. The analytical results were compared to concentration values obtained by HPLC and to pH-readings of the bioreactor broth media. The project was funded by the German Federal Ministry for Education and Research (BMBF).