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AKB: Biologische Physik
AKB 200: Poster Session II
AKB 200.27: Poster
Dienstag, 8. März 2005, 17:00–19:00, Poster TU C
In situ Synthesis of DNA Chips — •Thomas Naiser, Timo Mai, Wolfgang Michel, and Albrecht Ott — Physikalisches Institut, Universität Bayreuth, 95440 Bayreuth
DNA-Chips are biosensors for measuring gene activity on a genome wide scale. They have become an important tool in Biological Sciences. The underlying principle is the duplex formation of nucleic acids (hybridization), which is highly sequence-specific and can therefore be used to determine the composition of nucleic acid mixtures extracted from biological specimens. We have built a photolithographic micro-projection setup to manufacture high density DNA-Chips in a photochemically controlled synthesis process. Virtual lithography masks, generated with a Digital Micromirror Device (a spatial light modulator commonly used in video projectors), allow local control of the synthesis, so that we can produce an array of closely spaced spots (10-15 micron in size), each one containing a different sequence of single stranded DNA. The whole chip comprises up to 105 freely programmable sequences (15-25mers) on an area of 10 mm2 . We present results from hybridization assays performed to investigate the physics underlying DNA-Chip technology.