Berlin 2005 – scientific programme
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AKB: Biologische Physik
AKB 200: Poster Session II
AKB 200.41: Poster
Tuesday, March 8, 2005, 17:00–19:00, Poster TU C
A Simple Scheme for Rapid 3D Orientation Determination of the Emission Dipole of Single Molecules — •Johannes Hohlbein1 and Christian G. Hübner2 — 1Max Planck Institute of Microstructure Physics, Weinberg 2, 06120 Halle — 2Martin Luther University Halle-Wittenberg, Department of Physics, Hoher Weg 8, 06120 Halle
One of the unique features of single molecule absorption and emission is their anisotropy due to the well-defined transition dipole(s) for both processes allowing the determination of the molecule’s orientation. While polarization-resolved techniques are usually capable to detect only a projection of the transition dipole, several methods have been proposed in order to determine the full three-dimensional orientation. Here, we report on a new detection scheme that allows for a shot-noise limited determination of the emission dipole orientation utilizing an annular mirror, a polarizing beam splitter in conjunction with three detectors in a scanning confocal optical microscope.