Berlin 2005 – wissenschaftliches Programm

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AKB: Biologische Physik

AKB 200: Poster Session II

AKB 200.44: Poster

Dienstag, 8. März 2005, 17:00–19:00, Poster TU C

Kinetics of Solid-Phase DNA Hybridization — •Timo Mai, Thomas Naiser, Wolfgang Michel, Philipp Baaske, and Albrecht Ott — Physikalisches Institut, Universität Bayreuth, 95440 Bayreuth

Solid-phase hybridization of DNA oligonucleotides is of growing importance because of the advances in DNA microarray technology.

We apply two distinct strategies both commonly used in DNA microarray experiments: Immobilization of pre-fabricated oligonucleotides and light directed in-situ synthesis. We investigate kinetics of hybridization on glass substrates using evanescent field excitation and fluorescence labelling of oligonucleotides. A FRET technique is used to account for contributions of nonspecific adsorption to the substrate during the hybridization process. We discuss the impact of these different methods of immobilization on hybridization kinetics.