Berlin 2005 – scientific programme
Parts | Days | Selection | Search | Downloads | Help
AKB: Biologische Physik
AKB 200: Poster Session II
AKB 200.58: Poster
Tuesday, March 8, 2005, 17:00–19:00, Poster TU C
Electric field induced perturbation of the energy metabolism of living yeast cells — •Ch. Warnke1, T. Mair1, S.C. Mueller1, A. Reiher2, A. Kritschl2, H. Witte2, and A. Krost2 — 1Otto-von-Guericke Universität Magdeburg, Inst.Exp.Phy., Abt.Biophysik — 2Otto-von-Guericke Universität Magdeburg, Inst.Exp.Phy., Abt.Halbleiterepitaxie
Electric fields are often used for biophysical or biomedical treatment of biological cells, e.g. cell fusion or killing of cells. Despite these important applications, there are only a few data about the possible mechanisms that determine the electrosensitivity of biological cells. Since electrostimulation always induces depolarization of biomembranes, an impact of the energy metabolism is obvious due to the regeneration of electrochemical gradients by the expenditure of cellular energy. We have constructed a new electrical interface for local stimulation of biological cells with variable duration and amplitude. When applying short lasting electrical pulses to yeast cells, we find a direct response of the energy metabolism (measured by NADH-fluorescence) to these pulses. A sudden and fast decrease in NADH is followed by a slower recovery of the fluorescence signal. We attribute these changes to the immediate break down of ATP as a consequence of the regeneration of the membrane potential (ATPases) and the slower regeneration of ATP by glycolysis and respiration. We present a first kinetic analysis of this behaviour and basic characterization of the phenomenon.