Berlin 2005 – wissenschaftliches Programm
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AKB: Biologische Physik
AKB 200: Poster Session II
AKB 200.59: Poster
Dienstag, 8. März 2005, 17:00–19:00, Poster TU C
Characterization of Cellular Protein Distributions Using Karhunen-Lòeve Decompositon — •Ronny Straube1, Stefan C. Müller1, Ronald Koop2, and Walter Schubert2 — 1Abteilung Biophysik, Otto-von-Guericke-Universität Magdeburg, 39106 Magdeburg, Germany — 2MelTec GmbH, Leipziger Str. 44, 39120 Magdeburg, Germany
Immuno-fluorescence microscopy is a widely used technique to visualize the location of proteins on a cellular level. This method is based upon the interaction of fluorochrome labeled antibodies with antigens. In general, it is limited by the number of simultaneously usable fluorochrome labeled antibodies due to problems concerning the spectral separability of the fluorescence signals.
Recently, this limitation has been overcome by using a repetitive method (called MELK [1]) where many different antigens can be visualized on the same sample of cells. Thereby, it becomes now possible to study combinatorial patterns of protein distributions based on series of fluorescence intensity images of a high spatial resolution.
We use the Karhunen-Lòeve decomposition to characterize the cellular distributions of 18 human surface antigens on an ensemble of peripheral blood leukocytes (PBL’s). By analyzing the mode structure we find that some antigens tend to aggregate while others prefer to separate. We also investigate the reproducibility of the MELK patterns.
[1] Patentnummer (Deutschland): 197 09 348.5-52, W. Schubert, "Automatisches Multi-Epitop-Ligand-Kartierungsverfahren", MelTec GmbH, 1997