Berlin 2005 – wissenschaftliches Programm
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AKB: Biologische Physik
AKB 200: Poster Session II
AKB 200.8: Poster
Dienstag, 8. März 2005, 17:00–19:00, Poster TU C
Excitation beyond the monochromatic laser limit: Simultaneous 3-D confocal and multiphoton microscopy with a single white-light laser source. — •Daniel Koch1, Timo Betz1, Jörn Teipel2, Wolfgang Härtig3, Jochen Guck1, Josef Käs1, and Harald Giessen2 — 1Universität Leipzig, Fakultät f Physik und Geowiss., Linnéstr 5, 04103 Leipzig — 2Universität Bonn, Institut f Angewandte Physik, Wegelerstr 8, 53115 Bonn — 3Universität Leipzig, Paul Flechsig Institut f Hirnforschung, Jahnallee 59, 04109 Leipzig
Confocal and multiphoton microscopy are essential tools in modern life sciences. They allow fast and highly resolved imaging of a steadily growing number of fluorescence markers ranging from labeled antibodies and fluorescence proteins to quantum dots, used for the localization and quantitative detection of molecules within living cells and organisms. Up to now, only one physical limitation seemed to be unavoidable. Both confocal and multiphoton microscopy rely on lasers as excitation sources, and their monochromatic radiation allows only a limited number of simultaneously usable dyes. We have overcome this limitation by successfully replacing all excitation lasers in a standard confocal microscope with the pulsed 430 to 1300 nm white-light which is generated in a tapered silica fiber. With this easily reproducible method, simultaneous confocal and multiphoton microscopy was demonstrated. By developing a coherent and intense laser source with spectral properties comparable to a mercury lamp, we provide the flexibilty to excite any desired fluorophore combination.