Berlin 2005 – wissenschaftliches Programm

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AKB: Biologische Physik

AKB 25: Active Networks and Cell Motility

AKB 25.6: Vortrag

Freitag, 4. März 2005, 16:45–17:00, TU H2013

Optical Deformability as an Intrinsic Differentiation Marker for Stem Cells — •Stefan Schinkinger, Falk Wottawah, Bryan Lincoln, Frank Sauer, and Jochen Guck — Universität Leipzig, Abteilung Physik der Weichen Materie, Linnéstr. 5, 04103 Leipzig

Despite major efforts in stem cell research, there is no unequivocal molecular marker available for characterization and sorting of stem cells or their state of differentiation. We use a microfluidic Optical Stretcher to determine the material properties of individual cells as a non-invasive cell marker. The mechanical properties measured are mainly influenced by the cytoskeleton, a polymeric protein network in the cell. Because the cytoskeleton also plays a major role in the function of cells, with this technique we are able to follow cell progression through the various stages of differentiation. Experiments were performed with a promyelocytic leukemia cell line called HL-60. Comparison with mature neutrophils and experiments with different precursor cells support our hypothesis. As no labeling is required, preparation time of samples is reduced and the risk of activation or differentiation due to stress can be kept minimal. Especially for therapeutic applications of stem cells where labeling of any kind is prohibitive, characterization by optical deformability as an intrinsic marker provides a reasonable alternative.