Berlin 2005 – wissenschaftliches Programm
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AKB: Biologische Physik
AKB 30: Biomaterials
AKB 30.4: Vortrag
Freitag, 4. März 2005, 18:15–18:30, TU H2013
Nitric Oxide Production in Mechanosensitive Osteocytes — •Daisuke Mizuno1, Aviral Vatsa2, Theo H. Smit3, Jenneke Klein-Nulend2, Frederick C. MacKintosh1, and Christoph F. Schmidt1 — 1Dept. Physics, Vrije Universiteit, Amsterdam, NL — 2Dept. Oral Cell Biol., ACTA, Vrije Universiteit, Amsterdam, NL — 3Dept. Clin. Phys.&Informatics, Univ. Hospital, Vrije Universiteit, Amsterdam, NL
Osteocytes are a type of bone cells that are embedded in the bone matrix, and whose main function is believed to be mechanosensing. If bone is mechanically loaded, activated osteocytes produce nitric oxide (NO) which controls in a sophisticated control network the activity of other types of bone cells which deposit or resorb bone matrix. This single-cell level mechanosensing and chemical signaling is essential for bone repair and adaptation. In this study we 1) apply well characterized mechanical stimuli to single osteocytes and 2) quantify the resultant chemical signaling with NO at the single cell level. Results were: i) Intracellular NO increased up to about 10 µM after stimulation with forces on the order of 10 pN generating about 1% strain. ii) The cell membrane is not a significant diffusion barrier for NO. iii) Surrounding cells occasionally more than 100 microns away from a stimulated one were seen to react rapidly (within 10s) to the stimulation.