Berlin 2005 – wissenschaftliches Programm
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AKB: Biologische Physik
AKB 50: Imaging and Microscopy
AKB 50.4: Vortrag
Montag, 7. März 2005, 16:45–17:00, TU H2013
Infrared-spectroscopic mapping of a single virus by near-field microscopy — •Markus Brehm, Thomas Taubner, and Fritz Keilmann — MPI für Biochemie, 82152 Martinsried (München)
Infrared fingerprint spectroscopy has traditionally been a powerful tool for chemical and structural analysis, but because of diffraction could not solve problems requiring <5 µ m microscopic resolution.
Scattering scanning near-field optical microscopy (s-SNOM) overcomes this limit by exploiting the near-field coupling between a sharp tip and the sample, allowing resolutions of 20 nm even at mid-infrared wavelengths [1,2].
Here we show infrared-spectroscopic mapping of a single virus within the spectral range of the protein amide-I band (≈ 1600−1700 cm−1) demonstrating a resolution of ≈ 100 times better compared to conventional infrared microscopy. We therefore believe that this method can be of significant use to some problems in biology.
1. T. Taubner, R. Hillenbrand, and F. Keilmann, "Performance of visible and mid-infrared scattering-type near-field optical microscopes," Journal of Microscopy, vol. 210, pp. 311-314, 2003.
2. T. Taubner, R. Hillenbrand, and F. Keilmann, "Nanoscale polymer recognition by spectral signature in scattering infrared near-field microscopy", Applied Physics Letters, vol. 85 (22), 2004.