Berlin 2005 – wissenschaftliches Programm
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AKB: Biologische Physik
AKB 50: Imaging and Microscopy
AKB 50.8: Vortrag
Montag, 7. März 2005, 17:45–18:00, TU H2013
Single particle fluorescence microscopy enlights active and diffusive transport processes of nanoparticles in living cells — •Ralf Bausinger1, Katharina von Gersdorff2, Manfred Ogris2, Christoph Bräuchle1, Ernst Wagner2, and Andreas Zumbusch1 — 1LMU München, Department of Chemistry, Butenandtstr. 5-13, Building E, D-81377 München — 2LMU München, Department of Pharmacy, Butenandtstr. 5-13, Building D, D-81377 München
Biochemical experiments demonstrated the general applicability of cationic polyethyleneimine-DNA complexes for the delivery of genetic material into the cell nucleus. However only few mechanistic details about this transfection process are known so far. We use single-molecule sensitive fluorescence video microscopy for the tracking of individual nanoparticles inside the cell in combination with structured wide-field illumination for the imaging and three-dimensional reconstruction of labelled cellular structures. Our observations include the interaction of the polyplexes with parts of the cytoskeletton, namely the actin stress fibers and the microtubules. We further analyse the role of mitosis for the delivery of gene carriers into the cell nucleus.