Berlin 2005 – wissenschaftliches Programm

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AKB: Biologische Physik

AKB 55: Molecular Motors

AKB 55.5: Vortrag

Dienstag, 8. März 2005, 15:30–15:45, TU H2013

Filament depolymerisation by motor proteins — •Gernot Klein, Karsten Kruse, and Frank Jülicher — MPIPKS, Noethnitzerstr. 38, 01187 Dresden

Many active processes in cells are driven by highly specialized motor proteins, which interact with filaments of the cytoskeleton. Members of the Kin-13 kinesin subfamily are able to interact specifically with filament ends and induce depolymerisation of the filaments ends. Recent in vitro assays and single molecule studies have shown, that MCAK accumulates at both ends of stabilized microtubules and induces depolymerisation while at the same time MCAK molecules do not generate directed motion along the microtubules [1].

We analyse both, a stochastic model and a generic mean-field description of this process. We discuss conditions under which motors dynamically accumulate at the filament end. Such a dynamic accumulation occurs for processive cutting, which implies, that the motor can remain attached to the shrinking edge after subunit removal. For processive cutting, the depolymerisation speed as a function of the bulk motor concentration can exhibit several different types of behaviour, including the possibility of a dynamic instability. We discuss our results in relation to recent experiments.

[1]A.W. Hunter, et al., Mol. Cell 11, 445 (2003)