Berlin 2005 – wissenschaftliches Programm
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AKB: Biologische Physik
AKB 60: Membranes and Vesicles
AKB 60.7: Vortrag
Dienstag, 8. März 2005, 17:30–17:45, TU H2013
In situ Formation of Polyelectrolyte Multilayer Architectures with Embedded Lipids Studied by Neutron Reflectometry — •Thomas Gutberlet1, Christophe Delajon2,3, Rumen Krastev2,4, and Helmut Möhwald2 — 1Paul-Scherrer-Institut, Villigen, Switzerland — 2Max-Planck-Institut of Colloids and Interfaces, Golm, Germany — 3ISIS Universite Louis Pasteur, Strasbourg, France — 4Hahn-Meitner-Institut, Berlin, Germany
Biomimetic surfaces with tailored properties are major object of application oriented biophysical studies. Of particular interest are surface-grafted films of hydrophilic polymers which provide a biomimetic environment for membranes-spanning proteins and lipid-protein membranes. We have studied the coupling of phospholipids to fully hydrated polymer cushions as potential planar biomimetic model membrane. A DMPC lipid bilayer was supported by polyelectrolyte multilayers (PEM). Positively and negatively charged polymer sample terminations were considered. Using neutron reflectometry it was shown that, whereas positively charged terminated samples did not favour deposition of a lipid layer, negatively charged terminated samples allowed deposition of a lipid bilayer. In the latter case, formation of a PEM on the phospholipid layer was possible and the formation of a polymer/lipid/polymer complex sandwich like structure has been proven. the results will be presented and potential applications discussed.