Berlin 2005 – wissenschaftliches Programm
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AKB: Biologische Physik
AKB 80: Microfluidics
AKB 80.2: Vortrag
Mittwoch, 9. März 2005, 12:00–12:15, TU H2013
Surface coating strategies for poly(dimethylsiloxane) microchannels — •Wibke Hellmich1, Jan Regtmeier1, Stephan Altmann2, Dario Anselmetti1, and Alexandra Ros1 — 1Experimental Biophysics and Applied Nanosciences, Physics Faculty, Bielefeld University — 2BASF AG, Polymer Physics, Ludwigshafen
Control of the surface properties in microfluidic systems plays an important role for successful bioanalytical applications. Especially the unspecific interaction of apolar biomolecules with hydrophobic microchannel surfaces highly affects the analysis efficiency in poly(dimethylsiloxane) (PDMS) devices. Therefore, detailed knowledge and derivatisation strategies of the surface properties of PDMS microchannels are of great importance. In this work, coating strategies for poly(ethyleneoxide) (PEO) molecules of different chain lengths are presented for the control of the surface properties of PDMS.
Contact angle measurements as well as atomic force microscopy revealed homogenous immobilisation of covalently attached PEO silanes and adsorbed triblock-copolymer (F108) to the PDMS surface. For F108, two different adsorption mechanisms to PDMS for hydrophobic (untreated) and hydrophilic (oxygen plasma treated) surfaces were observed. PEO coated microchannels revealed significant reduction of the electroosmotic mobility depending on the PEO chain length. The application of these coatings for protein separations in PDMS microfluidic devices is currently under investigation.