Berlin 2005 – wissenschaftliches Programm

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AKB: Biologische Physik

AKB 85: Biosensors and Biohybrid Systems

AKB 85.1: Vortrag

Mittwoch, 9. März 2005, 12:30–12:45, TU H2013

Characterization of cell adhesion and cell properties on suspended nanoporous membranes — •Bernhard Wolfrum, Youlia Mourzina, Matthias Höller, and Andreas Offenhäusser — ISG2:Bioelectronic Signal Processing, Research Centre Jülich, 52425 Jülich, Germany

Suspended membranes of nanoporous materials like porous alumina and porous silicon facilitate the development of interesting biohybrid systems, particularly with regard to their good biocompatibility. For example, these materials can be used as carriers for artificial or natural cell membranes to study cell signalling in a bioelectronic hybrid system.

A close contact between porous structure and cell membrane is a precondition for the functionality of such a device. We therefore investigated the adhesion and morphology of cortical rat neurons and cells from cell lines on porous silicon and porous alumina membranes using scanning electron microscopy. The results were compared with the adhesion of cells to other substrates like silicon nitride and silicon oxide. Further, we characterized electrophysiological properties of adherent cells on porous structures using patch-clamp techniques. The adherent cells can thereby be chemically stimulated through the nanopores in the substrate.