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CPP: Chemische Physik und Polymerphysik

CPP 30: POSTER: Chemical physics

CPP 30.1: Poster

Dienstag, 8. März 2005, 16:30–18:30, Poster TU D

Nucleosomal repositioning studied with single molecule FRET spectroscopy — •Alexander Gansen, Florian Hauger, Katalin Toth, and Jörg Langowski — Deutsches Krebsforschungszentrum, Abteilung: Biophysik der Makromoleküle, Im Neuenheimer Feld 580, 69120 Heidelberg

Fluorescence resonance energy transfer (FRET) has proven to be an important tool to investigate conformational and structural dynamics in vivo and in vitro. We use a confocal Single Pair - FRET system to analyse molecules diffusing freely in solution. Particles are detected by means of single photon counting and subsequent burst selection. Test experiments on doubly labelled DNA oligomers are presented that show the abilities and the limitations of the method.

Recent experiments focus on the analysis of the structure and dynamics of reconstituted mononucleosomes. SpFRET verified the bulk measured FRET efficiency of 15-20