Berlin 2005 – scientific programme
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CPP: Chemische Physik und Polymerphysik
CPP 30: POSTER: Chemical physics
CPP 30.46: Poster
Tuesday, March 8, 2005, 16:30–18:30, Poster TU D
Photo- and Electrochemical Stability of Dye-Labelled Oligonucleotides Tethered to Au Surfaces — •Kenji Arinaga1,2, Ulrich Rant2, Marc Tornow2, Shozo Fujita1, Naoki Yokoyama1, and Gerhard Abstreiter2 — 1Fujitsu Laboratories Ltd., 10-1 Morinosato-Wakamiya, Atsugi 243-0197, Japan — 2Walter Schottky Institut, Technische Universitaet Muenchen, 85748 Garching, Germany
Fluorescence investigations represent a powerful and widespread method to study dye-labelled oligonucleotides tethered to surfaces. Recently, we have been introducing the dynamic electrical manipulation of short DNAs on a biased Au surface, employing optical measurements to probe the orientation of the surface grafted DNA strands [1]. While this technique opens many possibilities for fundamentals and applications, long-time stability is a crucial issue to carry out sophisticated studies.
In this contribution, we assess the photo- and electrochemical stability of the employed DNA layers under controlled substrate potentials. The beneficial influence of the metal surface in proximity to the dye on its photostability is elucidated. Further, the durability of the organic adlayer against varying substrate potentials is studied to achieve maximal manipulation efficiency along with negligible sample degradation.
As a result, it is demonstrated that electro-optical investigations of surface grafted DNA layers can be conducted with extraordinary long-time stability and persistent functionality.
[1] U. Rant et al., Nano Letters (in press)