Berlin 2005 – wissenschaftliches Programm
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CPP: Chemische Physik und Polymerphysik
CPP 9: SYMPOSIUM: Polymer networks and beyond: From molecular structure to materials and biological functions POSTER
CPP 9.4: Poster
Samstag, 5. März 2005, 16:45–18:45, Poster TU D
Protein Deformation of Lipid Hybrid Bilayer Membranes Studied by Sum Frequency Generation Vibrational Spectroscopy — •Andrew Doyle1,2,3, Joerg Fick1, Michael Himmelhaus1, Wolfgang Eck1,3, Irene Graziani4, Igor Prudovsky4,3, Michael Grunze1,3, Thomas Maciag4,3, and David Neivandt2,3 — 1University of Heidelberg, Department of Applied Physical Chemistry, INF 253, Heidelberg, Germany 69120 — 2University of Maine, Department of Chemical and Biological Engineering, Jenness Hall, Orono, ME USA, 04469 — 3Institute for Molecular Biophysics, The Jackson Laboratory, Bar Harbor, ME USA, 04609 — 4Center For Molecular Medicine, Maine Medical Center Research Institute, Scarborough, ME USA, 04704
Structural deformations of lipid hybrid bilayer membranes induced by signal peptideless (SPL) proteins have been studied for the first time using the inherently surface specific nonlinear optical technique of sum frequency generation vibrational spectroscopy. Specifically, deformations of 1,2 distearoylphosphatidylglycerol (DSPG) membranes induced by interaction with FGF-1, a SPL protein which is released as a function of cellular stress through a nonclassical pathway, have been investigated. FGF-1 was found to induce lipid alkyl chain deformations in previously highly ordered DSPG membranes at the extremely low concentration of 1 nM at 60 ∘C. The deformation process was shown to exhibit a degree of reversibility upon removal of the protein by rinsing with buffer solution.