DPG Phi
Verhandlungen
Verhandlungen
DPG

Berlin 2005 – scientific programme

Parts | Days | Selection | Search | Downloads | Help

MS: Massenspektrometrie

MS 1: Ionenfallen

MS 1.4: Talk

Friday, March 4, 2005, 11:15–11:30, HU 3088

Infrared spectroscopy of gas-phase proteins — •Gert von Helden1, Jos Oomens2, Nick Polfer2, David T. Moore2, Lex van der Meer2, Alan G. Marshall3, John R. Eyler4, and Gerard Meijer11Fritz-Haber-Institut der Max-Planck-Gesellschaft, Faradayweg 4-6, 14195 Berlin — 2FOM Institute for Plasma Physics, Edisonbaan 14, NL-3439 MN Nieuwegein — 3National High Magnetic Field Laboratory, 1800 E. Paul Dirac Drive, Tallahassee, FL 32310-4005, USA — 4Department of Chemistry, University of Florida , Gainesville, FL 32611-7200, USA

The combination of an FT-ICR mass spectrometer with an infrared (IR) free electron laser is used to record IR spectra of a 104 amino-acid protein in the gas phase as a function of its charge state. The spectra are observed to contain clearly resolvable bands in the amide I and II spectral region. Compared to solution, the amide I band is blue-shifted and the amide II band red-shifted, as expected for species in an environment with reduced hydrogen bonding. The band positions are suggestive of a mostly α-helical structure of the protein and their widths are comparable to those in solution, indicating a similar conformational distribution.

100% | Mobile Layout | Deutsche Version | Contact/Imprint/Privacy
DPG-Physik > DPG-Verhandlungen > 2005 > Berlin