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MS: Massenspektrometrie

MS 3: Cluster und Aerosole

MS 3.5: Gruppenbericht

Freitag, 4. März 2005, 15:15–15:45, HU 3088

Real-time characterization of individual bioaerosol particles with bioaerosol mass spectrometry — •Matthias Frank¹, James M. Birch¹, Abneesh Srivastava¹, Paul T. Steele¹, Keith R. Coffee¹, David P. Fergenson¹, Herbert J. Tobias¹, Maurice E. Pitesky², Vincent J. Riot¹, Sue I. Martin¹, Eric E. Gard¹, Carlito Lebrilla², Gregg A. Czerwieniec², Scott C. Russell², and Erica L. McJimpsey² — ¹Lawrence Livermore National Laboratory, Livermore CA 94551, USA — ²University of California Davis, Davis CA 95616, USA

We are developing a single-particle bioaerosol mass spectrometry system that can rapidly analyze individual biological particles (e.g. spores or vegetative bacteria) that are sampled directly from air into a mass spectrometer. Mass spectra from both positive and negative ions created by matrix-free or matrix-assisted laser desorption and ionization are recorded simultaneously and can be analyzed and classified in real-time. Our present system is capable of discriminating, particle by particle, between bacterial spores and other biological and non-biological background materials using the mass fingerprints obtained from those particles. We will describe recent results including the comprehensive identification of critical marker peaks from spores by isotope labeling, the reduction of the shot-to-shot mass spectral variability by laser profile conditioning, and the demonstration of sub-attomole detection limits for peptide standards. We will also discuss the prospects for potential future applications in the detection of respiratory diseases.