Berlin 2005 – wissenschaftliches Programm

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O: Oberflächenphysik

O 36: Postersitzung (Elektronische Struktur, Grenzfl
äche fest-flüssig, Halbleiteroberfl
ächen und -grenzfl
ächen, Nanostrukturen, Oberfl
ächenreaktionen, Teilchen und Cluster, Struktur und Dynamik reiner Oberfl
ächen)

O 36.16: Poster

Montag, 7. März 2005, 15:00–18:00, Poster TU F

Surface design for attachment of biomolecules by adaptive polymer surfaces — •Nikolay Houbenov¹, Alexander Sidorenko¹, Leonid Ionov¹, Sergiy Minko², and Manfred Stamm¹ — ¹Leibniz-Institut fuer Polymerforschung e.V., Hohe Str 6, 01069 Dresden, Germany — ²Clarkson University Potsdam, NY 13699

Surface chemical patterning can be successfully used to control the spatial position of proteins attachment. We applied chemical patterning on stimuli responsive polymer brushes, consisted of a non-polar hydrophobic polymer (polyisoprene (PI)) and a polar one (Poly(2-vynilpyridine) (P2VP)). By patterning the substrate via photo-crosslinking of PI, we were able to generate chemistry driven differences in the adsorption of Bovine Serum Albumin (BSA). The surface micropattern appeared and disappeared interchangeably between pH 4 and 7, caused by swelling/deswelling behavior of P2VP in non-irradiated regions. The adsorption experiments indicated that BSA adsorbs strongly at the non-polar hydrophobic PI parts, where attachment would be expected to occur primarily by hydrophobic interaction. For the hydrophilic P2VP parts (at low pH), protein adsorption appears to occur by polar interactions resulting in a weaker attachment and a different conformation.